Journal: Journal of Biomedical Science

Article Title: BMP-2 gene-fibronectin-apatite composite layer enhances bone formation

doi: 10.1186/1423-0127-18-62

Figure Lengend Snippet: BMP-2 gene expression levels and BMP-2 protein concentrations in in-vitro experiments . BMP2 gene expression levels in extracts from MC3T3-E1 cells (A) or C3H10T1/2 cells (B) cultured on EVOH-CP, EVOH-BMP, or EVOH-BMP-FB for 3 or 7 days. The empty columns indicate a 3-day culture and the solid columns a 7-day culture. (C) BMP-2 concentrations in the medium from cells cultured on EVOH-CP, EVOH-BMP or EVOH-BMP-FB for 7 days. The empty columns indicate the MC3T3-E1 cells and the solid columns the C3H10T1/2 cells. The values presented are the mean ± standard deviation. (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The tissues in the bone deficit were taken, BMP-2 gene expression was evaluated with real-time PCR using primers specific to human BMP-2 and BMP-2 concentrations in the tissues were assessed using the human/mouse/rat BMP-2 Quantikine ELISA kit (R&D Technologies Inc.).

Techniques: Expressing, In Vitro, Cell Culture, Standard Deviation

Journal: Journal of Biomedical Science

Article Title: BMP-2 gene-fibronectin-apatite composite layer enhances bone formation

doi: 10.1186/1423-0127-18-62

Figure Lengend Snippet: BMP-2 gene expression and BMP-2 protein concentrations in animal experiments . BMP-2 gene expression (A) and BMP-2 protein concentrations (B) were evaluated in bone defect tissue treated with HAP-CP, HAP-BMP or HAP-BMP-FB two weeks after the procedure. The values presented are the mean ± standard deviation (n = 3, *p < 0.05, **p < 0.01).

Article Snippet: The tissues in the bone deficit were taken, BMP-2 gene expression was evaluated with real-time PCR using primers specific to human BMP-2 and BMP-2 concentrations in the tissues were assessed using the human/mouse/rat BMP-2 Quantikine ELISA kit (R&D Technologies Inc.).

Techniques: Expressing, Standard Deviation

Journal: Polymers

Article Title: Tailored Polyelectrolyte Multilayer Systems by Variation of Polyelectrolyte Composition and EDC/NHS Cross-Linking: Controlled Drug Release vs. Drug Reservoir Capabilities and Cellular Response for Improved Osseointegration

doi: 10.3390/polym14204315

Figure Lengend Snippet: Absorbance in XTT assay relative to TCPS after 3 and 7 days on different cross-linked PEMs without BMP-2 ( A – C ) or loaded with BMP-2 ( D – F ). Error bars represent standard deviations of three independent experiments with three technical replicates each. Results of a one-way ANOVA test can be found in the in and .

Article Snippet: The initial loading capacity as well as the release of rhBMP-2 were measured using a Human/Murine/Rat BMP-2 Standard TMB ELISA Development Kit (PeproTech Germany, Hamburg, Germany), according to the manufacturer’s instructions.

Techniques: XTT Assay

Journal: Polymers

Article Title: Tailored Polyelectrolyte Multilayer Systems by Variation of Polyelectrolyte Composition and EDC/NHS Cross-Linking: Controlled Drug Release vs. Drug Reservoir Capabilities and Cellular Response for Improved Osseointegration

doi: 10.3390/polym14204315

Figure Lengend Snippet: Results of ALP activity measurements after 7 days of cultivation in logarithmic scale; ( A – C ) without BMP-2; ( D – F ) with BMP-2. Results of a one-way ANOVA test can be found in the in .

Article Snippet: The initial loading capacity as well as the release of rhBMP-2 were measured using a Human/Murine/Rat BMP-2 Standard TMB ELISA Development Kit (PeproTech Germany, Hamburg, Germany), according to the manufacturer’s instructions.

Techniques: Activity Assay

Journal: Polymers

Article Title: Tailored Polyelectrolyte Multilayer Systems by Variation of Polyelectrolyte Composition and EDC/NHS Cross-Linking: Controlled Drug Release vs. Drug Reservoir Capabilities and Cellular Response for Improved Osseointegration

doi: 10.3390/polym14204315

Figure Lengend Snippet: Absorbance of ARS assay on different cross-linked PEMs unloaded ( A – C ) or loaded with BMP-2 ( D – F ) after 21 days of cultivation in logarithmic scale. Error bars represent standard deviations of three independent experiments with three technical replicates each. Results of a one-way ANOVA test can be found in the in .

Article Snippet: The initial loading capacity as well as the release of rhBMP-2 were measured using a Human/Murine/Rat BMP-2 Standard TMB ELISA Development Kit (PeproTech Germany, Hamburg, Germany), according to the manufacturer’s instructions.

Techniques: